OSNA I. Estudio multicéntrico del Ganglio Centinela intraoperatorio en cáncer de mama basado en el procedimiento OSNA - Grupos de trabajo del GES

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OSNA I. Estudio multicéntrico del Ganglio Centinela intraoperatorio en cáncer de mama basado en el procedimiento OSNA

Coordinador: Laia Bernet Vegué

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Objetivos:

Molecular Diagnosis of Sentinel Lymph Nodes for Breast Cancer: One Step Ahead for Standardization. Laia, Bernet Vegué MD, PhD^*; Marcos, Martinez Benaclocha MD, PhD^*; Refael, Cano Muñoz MD, PhD^†; Francisco, Sevilla Chica MD, PhD^*; Jose, Torró MD^‡; Blai, Ballester Sapiña MD^§; Maria, De la Cámara de las Heras Juan BSc^∥
Abstract: Sentinel node (SN) pathologic analysis shows great limitations related to reproducibility and challenges pathologists with respect to methodology and standardization. Recently, a new, highly specific and sensitive molecular procedure has been developed, based on cytokeratin 19 messenger RNA amplification, which allows for fast and effective intraoperative evaluation of the SN. Our aim in this study was to compare 2 arms for intraoperative evaluation: one study arm with 478 cases in which an exhaustive histopathologic procedure was performed and the other study arm with 211 cases that were analyzed using the new molecular method. Our results indicate that both methods similarly distinguish between benign and malignant lymph nodes but the type of metastasis is better defined and stratified by the molecular method. We conclude that the molecular method should be recommended for SN diagnosis because besides its high sensitivity, specificity, and operating room adequacy, it may allow for standardization for SN evaluation.
Publicado en:
Diagnostic Molecular PathologyMarch 2011 - Volume 20 - Issue 1 - p 18–21

Diagnosis of the sentinel lymph node in breast cancer: a reproducible molecular method: a multicentric Spanish study
Laia Bernet, Rafael Cano,1 Marcos Martinez, Basilio Duen˜ as,2 Xavier Matias-Guiu,3 Lluisa Morell,1 Jose Palacios, Ricardo Rezola, Maria Robles-Frias, Irune Ruiz, Ana Velasco,Begoña Vieites, Francisco Sevilla, Jose Torro, Jose Medrano, Blai Ballester
Abstract
Aims: Standardization of the sentinel node (SN) as a our study. For trial 2, the timing of every procedural diagnostic tool has not yet been achieved, because the step was recorded in an electronic database in order to protocol for histopathological study is highly variable discern the time spent for each step, the total SN between centres. We compared the results of a new evaluation time and to identify areas of improvement. method with conventional histological tests and eval-In the second trial, after a learning period and feedback uated its feasibility for intra-operative evaluation, and on data recorded, we spent a mean of 31 min for the propose it as a method to standardize the sentinel node entire SN evaluation procedure.
Conclusion: Our multi-centric trial using the OSNA
Methods and results: Trial 1 included 181 cases; in asay for sentinel node evaluation in breast cancer parallel, 2-mm-thick sections of the SN were processed demonstrates that this is a highly sensitive, specific and alternately for histological analysis and for the one-step reproducible technique that allows for standardization nucleic acid amplification (OSNA) procedure. A final of the SN diagnostic procedure, a necessary, and until concordance of 99.45% was observed in the first trial of now unresolved, issue.
Publicado en: Histopathology 2011, 58, 863–869. DOI: 10.1111/j.1365-2559.2011.03836

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